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Full Moon BioSystems
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MACHEREY NAGEL
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Image Search Results
Journal: bioRxiv
Article Title: Angiotensin II Type 2 Receptor Potentiates Skeletal Muscle Satellite Cell Differentiation via the GSK3β/β-catenin Pathway
doi: 10.1101/2022.09.30.510328
Figure Lengend Snippet: (A) Phosphoprotein changes analyzed by PhosphoExplorer array in muscle satellite cells (SCs) transfected with AT2R or control siRNA, followed by induction of differentiation for 2 days. Log2 fold change values of AT2R siRNA-transfected cells compared to control are shown, with increased phosphorylation in red and decreased phosphorylation in blue. (B, C) IPA Canonical Pathway analysis (B) and Diseases and Functions Annotations (C) for the phosphoprotein changes with absolute log2 fold change values over 1 (i.e. >2 fold change). Bars indicate -log 10 p-value, and lines show the ratio of the proteins that were changed among the proteins associated with each pathway. The red and blue colors of the bar chart indicate activation z-score, and only the pathways with absolute z-scores higher than 2 (i.e. considered statistically significant). (D) Changes in phosphorylation levels in GSK3β (Ser216 and Ser9), and β-catenin (Tyr654, Thr41/Ser45, Ser37, Ser33 and Tyr489).
Article Snippet: The
Techniques: Transfection, Activation Assay
Journal: Brain, Behavior, & Immunity - Health
Article Title: Impact of broad-spectrum antibiotics on the gut–microbiota–spleen–brain axis
doi: 10.1016/j.bbih.2022.100573
Figure Lengend Snippet: Effects of ABX on spleen weight and spleen cell types A: Schedule of the experiment. ABX or water was injected as drinking water for 14 days. Fresh feces samples were collected in the morning of day 15. Subsequently, plasma, spleen, and cerebral cortex were collected in the same day. B: ABX induced a significant reduction in spleen weight in mice (Two-tailed unpaired Student’s T test, P < 0.0001). Representative photograph of spleen from the two groups. C: FACS analysis of spleen cell populations in all mice (Mann-Whitney test: 11b + Ly6c + neutrophils, P = 0.0207; NK1.1 + NK cells, P = 0.0207; F4/80 + macrophages, P = 0.0070; CD3 + CD8 + T cells, P = 0.002). D: Representative FACS dot plots of neutrophils, NK cells, macrophages, and CD8 + T cells. E: Spearman correlation analysis of spleen cell population and spleen weight in all mice. Values represent mean ± S.E.M. (CON: n = 8. ABX: n = 9). *P < 0.05, **P < 0.01, ***P < 0.001. N.S.: not significant.
Article Snippet: The following antibodies were used for immunofluorescence staining: anti-CD3-FITC (x40, cat# 100305: BioLegnd, San Diego, CA), anti-CD4-allophycocyanin (x100, cat# 17-0042-82: eBioscience, San Diego, CA), anti-CD8a-allophycocyanin (x100, cat# 553035: BD Bioscience),
Techniques: Injection, Two Tailed Test, MANN-WHITNEY
Journal: Brain, Behavior, & Immunity - Health
Article Title: Impact of broad-spectrum antibiotics on the gut–microbiota–spleen–brain axis
doi: 10.1016/j.bbih.2022.100573
Figure Lengend Snippet: Effects of ABX on spleen weight and spleen cell types A: Schedule of the experiment. ABX or water was injected as drinking water for 14 days. Fresh feces samples were collected in the morning of day 15. Subsequently, plasma, spleen, and cerebral cortex were collected in the same day. B: ABX induced a significant reduction in spleen weight in mice (Two-tailed unpaired Student’s T test, P < 0.0001). Representative photograph of spleen from the two groups. C: FACS analysis of spleen cell populations in all mice (Mann-Whitney test: 11b + Ly6c + neutrophils, P = 0.0207; NK1.1 + NK cells, P = 0.0207; F4/80 + macrophages, P = 0.0070; CD3 + CD8 + T cells, P = 0.002). D: Representative FACS dot plots of neutrophils, NK cells, macrophages, and CD8 + T cells. E: Spearman correlation analysis of spleen cell population and spleen weight in all mice. Values represent mean ± S.E.M. (CON: n = 8. ABX: n = 9). *P < 0.05, **P < 0.01, ***P < 0.001. N.S.: not significant.
Article Snippet: The following antibodies were used for immunofluorescence staining: anti-CD3-FITC (x40, cat# 100305: BioLegnd, San Diego, CA), anti-CD4-allophycocyanin (x100, cat# 17-0042-82: eBioscience, San Diego, CA), anti-CD8a-allophycocyanin (x100, cat# 553035: BD Bioscience), anti-NK1.1-PE (x100, cat# 553165: BD Bioscience),
Techniques: Injection, Two Tailed Test, MANN-WHITNEY